Anti-BUB3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43684

Core Product Specifications and Parameters

Biological Background: BUB3 Function and Localization

• Mitotic checkpoint protein BUB3 is a key component of the spindle-assembly checkpoint, ensuring accurate chromosome segregation.
• It promotes the formation of stable end-on bipolar kinetochore-microtubule attachments, which is essential for proper chromosome alignment.
• BUB3 is required for the kinetochore localization of BUB1, forming the BUB1/BUB3 complex that inhibits the anaphase-promoting complex/cyclosome (APC/C) by phosphorylating CDC20.
• The protein also regulates chromosome segregation during oocyte meiosis, highlighting its role in germ cell division.
• Subcellular localization includes the nucleus, and it specifically localizes to the centromere and kinetochore regions of chromosomes during mitosis.
• Post-translational modifications such as acetylation, phosphorylation, and ubiquitination modulate its function and stability.
• BUB3 belongs to the WD repeat protein family and is subject to alternative splicing, though specific isoforms are not fully characterized.

Experimental Guidance and Technical Tips

• For western blotting, consider using whole-cell lysates from human, mouse, or rat cell lines; the predicted molecular weight is 37 kDa.
• In immunofluorescence/immunocytochemistry, validate fixation and permeabilization conditions to preserve kinetochore-associated staining patterns.
• The immunogen covers the full-length protein, so the antibody may exhibit broad reactivity across species; however, titration experiments are recommended to determine optimal dilutions.
• ELISA performance should be verified using the recombinant immunogen or cell extracts as coating antigen.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories integrated access to a wide range of IVD raw materials, including validated antibodies, bulk ancillary reagents, and rare target sourcing solutions. This anti-BUB3 polyclonal antibody supports investigations into spindle assembly checkpoint mechanisms and chromosome segregation, complementing our portfolio for cell cycle and oncology research. Explore our one-stop supply capabilities to streamline your workflow from concept to clinic.