Anti-BUB3 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O43684

Core Product Specifications and Parameters

Biological Background: BUB3 Function and Localization

• BUB3 (Mitotic checkpoint protein BUB3) is a ~37 kDa protein encoded by the BUB3 gene, with multiple isoforms produced through alternative splicing.
• It plays a dual role in the spindle-assembly checkpoint, both signaling and promoting correct kinetochore-microtubule (K-MT) attachments to ensure chromosome segregation fidelity.
• BUB3 forms a complex with BUB1 to inhibit the anaphase-promoting complex/cyclosome (APC/C) by phosphorylating its activator CDC20, thereby delaying anaphase onset when the checkpoint is active.
• The BUB1/BUB3 complex also phosphorylates MAD1L1, further contributing to mitotic arrest until all chromosomes achieve proper bipolar attachment.
• BUB3 is essential for the kinetochore localization of BUB1 and regulates chromosome segregation during oocyte meiosis.
• Subcellularly, BUB3 localizes to the nucleus and concentrates at centromeres and kinetochores on chromosomes during mitosis.
• Post-translational modifications include acetylation, ADP-ribosylation, phosphorylation, and ubiquitination, reflecting its dynamic regulation during the cell cycle.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human BUB3; the antibody is likely to recognize full-length BUB3 and any N-terminally conserved isoforms.
• For Western blotting, the predicted band is at ~37 kDa; due to potential post-translational modifications, additional bands may be observed; validate specificity using appropriate positive and negative controls.
• In IHC-P and IF/ICC, optimize antigen retrieval and fixation/permeabilization conditions for your specific tissue or cell type; consider using known kinetochore markers as co-stains to confirm localization.
• ELISA applications may require titration of both capture and detection reagents; no absolute OD values are provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner offering diagnostic manufacturers and researchers one-stop access from concept to clinic. This anti-BUB3 monoclonal antibody supports spindle-assembly checkpoint and cell cycle investigations. Beyond antibodies, CamelBio supplies validated antibody pairs, bulk ancillary reagents, and can source rare targets to accelerate your assay development.