Anti-BTN3A3 Rabbit Polyclonal Antibody for WB, ELISA - O00478

Core Product Specifications and Parameters

Biological Background: BTN3A3 Function and Localization

• BTN3A3, also designated BTF3 or BTN3.3, is a member of the butyrophilin subfamily 3, characterized by immunoglobulin-like domains and a single transmembrane region.
• It plays a role in T-cell responses during the adaptive immune response, contributing to immune surveillance and regulation.
• The protein is localized at the cell membrane.
• Expression is detected in peripheral blood mononuclear cells, T-cells, spleen, and lymphocytes at the protein level.
• Alternative splicing generates multiple transcript variants.
• Post-translational modifications include glycosylation, disulfide bond formation, and a signal peptide.
• The predicted molecular weight is 65,002 Da, consistent with the observed band at approximately 65 kDa.
• Three-dimensional structural data are available, supporting functional studies of its immunoglobulin domains.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 510–584), likely intracellular; antibodies may detect both full-length and processed forms.
• For Western blotting, expect a band near 65 kDa under reducing conditions; validate expression in relevant human, mouse, or rat lymphoid tissues or cell lines.
• In ELISA, use recombinant BTN3A3 protein or cell lysates as coating antigen; consider pairing with detection antibodies for sandwich assays.
• Because of homology within the BTN3 family, specificity should be verified with recombinant proteins of BTN3A1 and BTN3A2 if cross-reactivity is a concern.
• For T-cell functional studies, confirm target expression in isolated primary T-cells or PBMCs before downstream assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects involving butyrophilin family targets or adaptive immunity research, we offer sourcing solutions for rare targets and custom development services. This BTN3A3 rabbit polyclonal antibody is one example of our commitment to providing reliable tools for immune-diagnostic and therapeutic development.