Anti-BTN2A1 Rabbit Polyclonal Antibody for WB and ELISA - Q7KYR7

Core Product Specifications and Parameters

Biological Background: BTN2A1 Function and Localization

• BTN2A1 (Butyrophilin subfamily 2 member A1) is encoded by the BTN2A1 gene (Gene ID: 11120) and is also known by the aliases BTF1, BT2.1, and BTN2.1.
• It is a membrane protein, as indicated by the UniProt keywords Membrane, Signal, and Transmembrane helix.
• The protein contains an immunoglobulin-like domain and coiled-coil regions, characteristic of the butyrophilin family (keywords: Immunoglobulin domain, Coiled coil).
• Post-translational modifications include glycosylation and disulfide bond formation (keywords: Glycoprotein, Disulfide bond).
• Subcellular localization: Membrane (single-pass type I membrane protein).
• Tissue expression is broad, with high levels in brain, bone marrow, small intestine, muscle, spleen, and pancreas; moderate expression in lung, liver, and kidney.
• The precise function of BTN2A1 has not been annotated in UniProt.

Experimental Guidance and Technical Tips

• The immunogen (aa 30-240) corresponds to the N-terminal extracellular region; this design suggests the antibody may recognize the native, cell-surface form of BTN2A1.
• For Western blot, the predicted molecular weight is approximately 60 kDa; glycosylation may cause the band to migrate at a slightly higher apparent mass.
• In ELISA, consider using recombinant extracellular domain or lysates from tissues with high BTN2A1 expression (e.g., brain, bone marrow) as the coating antigen.
• Optimal antibody dilution should be determined experimentally; typical starting dilutions for polyclonal antibodies are 1:500–1:2000 for WB and 1:1000–1:5000 for ELISA.
• Include appropriate positive and negative controls, such as BTN2A1-overexpressing cells or knockdown samples, to confirm band identity.

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