Anti-BTN2A1 Rabbit Monoclonal Antibody for WB, ELISA - Q7KYR7

Core Product Specifications and Parameters

Biological Background: BTN2A1 Function and Localization

• BTN2A1 (Butyrophilin subfamily 2 member A1) is a single-pass type I transmembrane protein encoded by the BTN2A1 gene (aliases: BT2.1, BTF1, BTN2.1, DJ3E1.1, BK14H9.1) on human chromosome 6.
• The full-length protein consists of an N-terminal signal peptide, two immunoglobulin-like domains (IgV and IgC1), a transmembrane helix, and a cytoplasmic coiled-coil domain (UniProt Keywords: Immunoglobulin domain, Coiled coil, Transmembrane helix, Glycoprotein, Disulfide bond).
• BTN2A1 is primarily localized to the plasma membrane (SUBCELLULAR LOCATION: Membrane).
• Tissue expression is broad and highest in the brain, bone marrow, small intestine, muscle, spleen, and pancreas; moderate expression is observed in lung, liver, and kidney.
• Post-translational modifications include N-linked glycosylation and intrachain disulfide bonds, which are important for protein folding and stability.
• Alternative splicing generates multiple transcript variants, potentially producing isoforms with distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 100 residues (extracellular region), so the antibody likely recognizes an epitope in the IgV-like domain. For Western blotting, expect a specific band around 60 kDa in human cell lysates; reducing conditions are recommended. Validate using positive controls such as HeLa, THP-1, or Jurkat cells.
• In ELISA, the antibody can be employed as capture or detection reagent for human BTN2A1. Titration experiments are necessary to determine the optimal coating concentration and detection dilution.
• The antibody cross-reacts with human BTN2A1; cross-reactivity with other butyrophilin family members (e.g., BTN2A2, BTN3A1) has not been tested and should be verified if multiplexed assays are planned.
• For functional studies, note that the antibody binds the extracellular domain; therefore, it may interfere with ligand–receptor interactions. Include appropriate isotype controls.
• If weak signals are obtained, consider using high-expression cell models (e.g., BTN2A1-overexpressing HEK293T) or enrichment of membrane fractions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a specialized one-stop sourcing bridge for IVD raw materials, supporting diagnostic manufacturers and research laboratories from target discovery to assay commercialization. Our catalog includes validated monoclonal antibodies like anti-BTN2A1 rabbit mAb, along with optimized antibody pairs, bulk ancillary reagents, and custom development of rare target materials. By streamlining procurement and technical support, CamelBio accelerates the translation of butyrophilin-family biomarkers into high-performance immunoassays.