Anti-BTK Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q06187

Core Product Specifications and Parameters

Biological Background: BTK Function and Localization

• Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Related references: PMID:19290921
• Upon B-cell receptor engagement, phosphorylates PLCG2 in cooperation with BLNK, triggering calcium mobilization and PKC activation. Related references: PMID:11606584
• Functions as a component of the Toll-like receptor (TLR) pathway; critical for TLR9 activation and signaling in splenic B-cells. Related references: PMID:16517732, PMID:17932028
• Induces NF-kappa-B transcriptional activity, linking TLR8 and TLR9 pathways to gene expression regulation. Related references: PMID:19290921
• Acts as an activator of NLRP3 inflammasome assembly by mediating NLRP3 phosphorylation. Related references: PMID:34554188
• Phosphorylates transcription factor GTF2I, which translocates to the nucleus to modulate gene expression in response to BCR signaling. Related references: PMID:9012831
• Required for the formation of functional ARID3A DNA-binding complexes, with no evidence of direct DNA binding. Related references: PMID:16738337
• Localizes to the cytoplasm, cell membrane, nucleus, and membrane rafts; predominantly expressed in B-lymphocytes.

Experimental Guidance and Technical Tips

• The immunogen peptide lies within residues 560–659 near the C‑terminus; consider this when assessing detection of isoforms or cleavage products.
• For Western blotting, expect a band at ~76 kDa; as with all antibodies, validate in your specific sample and control lysates.
• For IHC-P and IF-P, optimize antigen retrieval conditions and primary antibody dilution for your tissue type.
• The antibody cross-reacts with mouse BTK, supporting translational and comparative studies; always confirm expression in your model system.
• ELISA performance may require optimization of coating conditions and detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a complete spectrum of IVD raw materials, streamlining your procurement from research to diagnostic development. This anti-BTK monoclonal antibody exemplifies our commitment to supplying thoroughly characterized reagents for B-cell signaling and immunodeficiency research. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets, CamelBio is your trusted partner.