Anti-BTG3 Polyclonal Antibody for WB, ELISA - Q14201

Core Product Specifications and Parameters

Biological Background: BTG3 Function and Localization

• Protein BTG3, also known as abundant in neuroepithelium area protein (ANA) or Tob5, is a member of the BTG/Tob family of antiproliferative factors.
• Overexpression of BTG3 impairs serum-induced cell cycle progression from the G0/G1 to S phase, suggesting a role as a negative regulator of cell proliferation.
• BTG3 is ubiquitously expressed, with high levels in the ventricular zone of the developing central nervous system and in adult tissues including ovary, testis, prostate, thymus, and lung.
• The BTG3 gene undergoes alternative splicing, producing multiple transcript variants.
• The protein has been identified in large-scale proteomics studies and is included in the human reference proteome.
• The calculated molecular weight of BTG3 is approximately 29 kDa (29116 Da), consistent with the observed ~29 kDa band.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated against a recombinant protein spanning amino acids 150–250 of human BTG3; verify reactivity in the relevant sample system prior to use.
• For Western blot, a specific band near 29 kDa is expected; consider using molecular weight markers and, if available, a knockdown or knockout control to confirm specificity.
• In ELISA, titrate the antibody against the immunogen or a known BTG3-containing sample to determine optimal working concentration.
• Cross-reactivity is reported for human BTG3; for other species, sequence homology should be examined and validity assessed experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research laboratories, providing one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This BTG3 polyclonal antibody exemplifies our commitment to supporting cell cycle and developmental biology research. Beyond catalog products, we offer rare target sourcing and technical services to accelerate your assay development from concept to clinic.