Anti-BTG1 Polyclonal Antibody for WB, IF/ICC, ELISA - P62324

Core Product Specifications and Parameters

Biological Background: BTG1 Function and Localization

• Protein BTG1, also known as B-cell translocation gene 1 protein, is encoded by the BTG1 gene in humans.
• BTG1 functions as an anti-proliferative protein, negatively regulating cell growth.
• It is classified as a proto-oncogene and is associated with chromosomal rearrangements, linking it to oncogenic processes.
• BTG1 is a phosphoprotein, indicating that its activity may be modulated by phosphorylation.
• The protein has a molecular weight of approximately 19.2 kDa (UniProt), consistent with the observed 19 kDa in experimental settings.

Experimental Guidance and Technical Tips

• The immunogen, covering amino acids 22–171 of human BTG1, encompasses most of the protein; this broad coverage may improve detection across potential isoforms or cleavage products.
• For Western blotting, expect a band near 19 kDa; validate in human cell lysates given the confirmed human reactivity.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions, as BTG1 localization may be context-dependent.
• In ELISA applications, the antibody can detect recombinant or native BTG1; consider pairing with a capture antibody for quantitative sandwich immunoassays.
• Since BTG1 is a low-abundance regulatory protein, enrichment strategies or sensitive detection methods may be necessary for clear signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with streamlined access to IVD raw materials, from concept to clinic. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For targets like BTG1—involved in cell proliferation and cancer—we facilitate sourcing of rare-specificity raw materials, accelerating your assay development and commercialization.