Anti-BTF3 Rabbit Polyclonal Antibody for WB, ELISA - P20290

Core Product Specifications and Parameters

Biological Background: BTF3 Function and Localization

• Protein identity: BTF3, also known as NAC-beta (Nascent polypeptide-associated complex subunit beta) and RNA polymerase B transcription factor 3, is encoded by the BTF3 gene in humans.
• Core function in protein targeting: When associated with NACA, BTF3 prevents incorrect targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). It binds to nascent polypeptide chains emerging from the ribosome and blocks their interaction with the signal recognition particle (SRP).
• Transcription regulation: BTF3 acts as a general transcription factor and can form a stable complex with RNA polymerase II, required for the initiation of transcription.
• Subcellular localization: BTF3 is found in both the cytoplasm and the nucleus, consistent with its dual roles in protein transport and transcription.
• Post-translational modifications: The protein is subject to phosphorylation and methylation, as indicated by UniProt keywords.
• Alternative splicing: BTF3 has multiple isoforms (e.g., BTF3a, BTF3b) due to alternative splicing, which may contribute to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering residues 50-150 of human BTF3; this region is conserved across the reported isoforms and species, suggesting broad reactivity.
• For Western blot analysis, consider testing nuclear and cytoplasmic fractions to confirm the expected subcellular localization pattern (~22 kDa band).
• For ELISA, validation with recombinant BTF3 protein or peptide blocking experiments may be helpful to assess specificity.
• Optimize antibody dilution and incubation conditions in the relevant sample system to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-BTF3 antibody. Our portfolio covers bulk ancillary reagents and custom sourcing for specialized targets, including transcription factors and NAC complex components, to support your development from discovery to clinical application.