Anti-BTBD7 Rabbit Polyclonal Antibody for WB, ELISA - Q9P203

Core Product Specifications and Parameters

Biological Background: BTBD7 Function and Localization

• BTB/POZ domain-containing protein 7 (BTBD7), also known as FUP1, is encoded by the BTBD7 gene (GeneID: 55727) and belongs to the BTB/POZ domain family.
• Functions as a mediator of epithelial dynamics and organ branching by promoting cleft progression, locally inducing SNAIL2 expression and suppressing E-cadherin to alter cell morphology and reduce adhesion.
• Subcellular localization: Primarily located in the nucleus, consistent with its role in transcriptional regulation of epithelial plasticity.
• The protein is subject to alternative splicing, phosphorylation, and myristoylation, which may modulate its activity and interactions during development.
• Annotated as a developmental protein and phosphoprotein, highlighting its involvement in morphogenesis and signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 5–75) of human BTBD7, targeting a sequence conserved across Human, Mouse, and Rat—consider this epitope location when designing blocking peptides or validation strategies.
• For Western blot (WB), consider starting with a recommended dilution range and validate in the relevant species (Human, Mouse, Rat) due to cross‑reactivity; the expected band size is ~126 kDa.
• For ELISA applications, assess coating efficiency and detection sensitivity using recombinant BTBD7 or cell lysates from epithelial tissues where BTBD7 is expressed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high‑quality IVD raw materials to diagnostic manufacturers and research institutes, offering validated antibody pairs, optimized polyclonal antibodies like this anti‑BTBD7, bulk ancillary reagents, and tailored sourcing for rare targets. Partner with us to accelerate your epithelial biology and developmental assay development from concept to clinic.