Anti-Btbd11 Rabbit Polyclonal Antibody for WB and ELISA - Q6GQW0

Core Product Specifications and Parameters

Biological Background: Btbd11 Function and Localization

• Btbd11, also known as Ankyrin repeat- and BTB/POZ domain-containing protein 3, is encoded by the Abtb3 (Btbd11) gene in mouse.
• It is specifically expressed in cortical and hippocampal inhibitory interneurons, as highlighted by its tissue specificity.
• The protein localizes to the membrane and is concentrated at glutamatergic (excitatory) synapses, where it supports cell type-specific synaptic function.
• Btbd11 contains an ANK repeat and a BTB/POZ domain, as indicated by its keywords.
• The protein is predicted to have transmembrane helices, consistent with its membrane localization.
• Alternative splicing produces multiple isoforms, which may vary in domain composition and function.
• The UniProt reference proteome includes this protein with a calculated molecular weight of approximately 121.6 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal amino acids 1-120; consider possible detection of N-terminal isoforms or cleavage products.
• For Western blotting, a band at ~122 kDa is expected; pre-stained markers in this high molecular weight range are recommended.
• Brain tissue lysates may require optimized extraction conditions for membrane proteins; consider using RIPA buffer or similar.
• Validate antibody specificity in relevant cell lines or tissues, such as mouse brain, using appropriate controls.
• For ELISA, determine optimal coating antigen concentration and antibody dilution by checkerboard titration.
• Cross-reactivity with human and rat indicates potential for multi-species studies, but validate in each species independently.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio streamlines IVD raw material procurement, offering validated antibody pairs, recombinant proteins, and ancillary reagents for neuroscience and rare target research.
• For synaptic targets like Btbd11, we can source or develop custom monoclonal and polyclonal antibodies, ensuring batch-to-batch consistency.
• Our end-to-end support—from antigen design to bulk supply—helps diagnostic manufacturers accelerate development of high-performance assays.