Anti-BTAF1 Rabbit Polyclonal Antibody for WB, IP, ELISA, ChIP - O14981

Core Product Specifications and Parameters

Biological Background: BTAF1 Function and Localization

• BTAF1, also known as TATA-binding protein-associated factor 172 (TAF-172), MOT1, or TAF(II)170, is an ATP-dependent helicase that functions as a key regulator of transcription.
• It associates with the TATA-binding protein (TBP) and catalyzes the ATP-dependent removal of TBP from the TATA box, thereby modulating transcription initiation.
• The protein contains conserved ATP-binding and helicase domains, classifying it as a member of the SWI2/SNF2 family of DNA-dependent ATPases.
• Alternative splicing of the BTAF1 gene generates multiple transcript variants, potentially contributing to functional diversity.
• Subcellularly, BTAF1 localizes exclusively to the nucleus, consistent with its role in transcriptional regulation.
• Post-translational modifications include phosphorylation, as indicated by proteomics data, which may influence its activity or interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 1600–1849), which may contribute to specific detection of the full-length protein or certain splice variants.
• For Western blot, a band of approximately 207 kDa is expected; use an appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunoprecipitation experiments, verify co-precipitation of TBP to confirm native complex formation.
• For ChIP assays, consider cross-linking optimization to capture transient TBP-DNA interactions regulated by BTAF1.
• Cross-reactivity with rat has been reported; however, validate in your specific sample system and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. This anti-BTAF1 polyclonal antibody is part of our offering for transcription and gene regulation studies. We also provide validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development from concept to clinic.