Anti-BSU1 Rabbit Polyclonal Antibody for WB and ELISA - Q9LR78

Core Product Specifications and Parameters

Biological Background: BSU1 Function and Localization

• BSU1 is a serine/threonine-protein phosphatase that functions as a positive regulator of brassinosteroid (BR) signaling in Arabidopsis thaliana. Related references: PMID:14977918, PMID:21855796
• It dephosphorylates and activates the transcription factor BES1, thereby modulating BR-responsive gene expression. Related references: PMID:14977918
• The phosphatase inactivates the negative BR signaling regulator ASK7/BIN2 by dephosphorylating it at Tyr-200. Related references: PMID:21855796
• BSU1 contains Kelch repeats and a protein phosphatase domain, requiring manganese as a metal cofactor for catalytic activity.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• Expression is predominantly observed in young, elongating tissues such as the base of hypocotyls, cotyledon tips, root vasculature, and lateral root emergence sites, with enrichment in vascular tissues.
• BSU1 is classified as a phosphoprotein and a member of the serine/threonine phosphatase family.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a synthetic peptide spanning amino acids 694–793 of Arabidopsis BSU1 (NP_171844.6). For applications such as ELISA, ensure that the coating antigen or detection setup is compatible with this region.
• In Western blotting, BSU1 migrates at approximately 88 kDa. Consider using protein extracts from young Arabidopsis tissues (e.g., seedlings or elongating stems) where BSU1 expression is high. Validate with appropriate positive and negative controls.
• Since BSU1 is a nuclear protein, confirm subcellular localization studies in relevant plant tissue systems if immunofluorescence applications are considered.
• For all applications, standard optimization of antibody concentration and blocking conditions is advised to maximize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized provider of IVD raw materials, CamelBio offers reliable access to validated antibodies, bulk reagents, and ancillary supplies for diagnostic and research applications. This anti-BSU1 polyclonal antibody supports plant signaling research, particularly brassinosteroid pathway studies, and aligns with our commitment to supplying high-quality molecular tools. Whether you need optimized antibody pairs, monoclonal alternatives, or rare-target raw materials, our expertise in one-stop sourcing and technical consultation can accelerate your project from concept to clinic.