Anti-BSND Rabbit Polyclonal Antibody for WB, ELISA - Q8WZ55

Core Product Specifications and Parameters

Biological Background: Barttin Function and Localization

• Barttin is a regulatory subunit of the anion-selective chloride channels CLCNKA and CLCNKB. These heteromeric channels are essential for basolateral chloride conductance along the nephron, enabling urine concentration and systemic acid-base homeostasis, and in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing. Related references: PMID:11734858 PMID:12111250 PMID:12574213
• Barttin acts as a chaperone that allosterically regulates proper sorting of CLCNKA:BSND and CLCNKB:BSND channels to the basolateral plasma membrane domain and mediates the constitutive opening of channel common gates. Related references: PMID:11734858 PMID:12111250 PMID:12574213
• Encoded by the BSND gene, mutations in barttin are associated with Bartter syndrome type IV, characterized by renal salt wasting and sensorineural deafness.
• Barttin localizes specifically to the basolateral cell membrane in expressing tissues.
• It is predominantly expressed in the kidney, within specific nephron segments, and in the stria vascularis of the inner ear.
• Post-translational modifications include palmitoylation and phosphorylation, which may regulate its function and membrane association.
• The protein has a molecular weight of approximately 35 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 54-320 of human BSND, covering the majority of the protein. As a polyclonal antibody, it likely recognizes multiple epitopes; validate specificity in your sample system.
• For Western blot, a band around 35 kDa is expected. Use appropriate loading controls and secondary antibodies, and verify the band size with molecular weight markers.
• In ELISA applications, consider potential higher background common with polyclonal antibodies; optimize blocking and washing steps to improve signal-to-noise ratio.
• Reported cross-reactivity includes human, mouse, and rat; if working with other species or specific tissue types, confirm reactivity in your experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This BSND antibody exemplifies our capability to supply high-quality polyclonal antibodies for renal physiology and hearing research. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to accelerate your assay development.