Anti-BRWD1 Polyclonal Antibody for WB, ELISA - Q9NSI6

Core Product Specifications and Parameters

Biological Background: BRWD1 Function and Localization

• BRWD1 (Bromodomain and WD repeat-containing protein 1), also called WDR9 or DCAF19, is a large multi-domain protein containing a bromodomain and multiple WD40 repeats.
• It is predicted to act as a transcriptional activator and may participate in chromatin remodeling, possibly through its bromodomain (By similarity).
• BRWD1 is required for proper cell morphology and cytoskeletal organization (PubMed:21834987). Related references: PMID:21834987
• The protein localizes to the cytoplasm, nucleus, cilium membrane, and flagellum axoneme, suggesting dual roles in gene regulation and ciliary/flagellar function.
• BRWD1 is ubiquitously expressed, with specific detection in respiratory epithelial cells and testis spermatozoa, highlighting its relevance in motile cilia and flagella.
• Post-translational modifications include phosphorylation, and mutations in BRWD1 are associated with primary ciliary dyskinesia, a ciliopathy.
• Multiple isoforms are generated through alternative splicing, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-120) of human BRWD1, which may confer specificity to the antibody.
• For Western blotting (WB), consider using a high-molecular-weight protein transfer protocol (e.g., low methanol, extended transfer time) to ensure efficient blotting of the 263 kDa target.
• In ELISA applications, this polyclonal antibody can be employed as a detection reagent; proper coating antigen and blocking conditions should be optimized for best results.
• Due to the polyclonal nature, lot-to-lot consistency should be verified, and appropriate secondary antibody controls are recommended.
• Cross-reactivity with mouse BRWD1 is indicated, but validation in mouse-derived systems is advised before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier supporting diagnostic manufacturers and research institutions. This anti-BRWD1 polyclonal antibody serves as a reliable tool for investigating chromatin remodeling and ciliary dysfunction. Beyond individual reagents, we offer validated antibody pairs, bulk ancillary reagents, and rare-target sourcing to streamline your development workflow from concept to clinic.