Anti-BRRF2 Monoclonal Antibody for WB, IF/ICC, ELISA - P03210

Core Product Specifications and Parameters

Biological Background: Tegument protein BRRF2 Function and Localization

• BRRF2 is a tegument protein of Epstein-Barr virus (HHV-4), localized in the virion tegument layer.
• It plays a key role in viral immune evasion by inhibiting the host cGAS-mediated DNA sensing pathway, thereby blocking dsDNA-triggered induction of antiviral genes.
• The protein interacts with and suppresses host cGAS, limiting cGAS-DNA phase separation and downstream signaling.
• Subcellularly, BRRF2 localizes to the virion tegument, host early endosomes, and is also secreted, suggesting multiple functional compartments.
• It is characterized as a phosphoprotein, likely undergoing post-translational modifications that regulate its activity.
• The protein has a molecular weight of approximately 56 kDa (UniProt: 56955 Da).

Experimental Guidance and Technical Tips

• The antibody was raised against recombinant BRRF2 protein; it is recommended to validate reactivity with native protein in EBV-infected cell lysates or purified virions.
• For WB, consider using whole-cell lysates from lytically induced EBV-positive cell lines; the expected band is at ~56 kDa.
• In IF/ICC, BRRF2 may show punctate cytoplasmic and/or virion-associated staining; co-staining with early endosome markers can confirm localization.
• For ELISA, the antibody can be used for direct or capture detection of recombinant or native BRRF2; titration is advised for optimal signal-to-noise ratio.
• Cross-reactivity with other herpesvirus homologs should be tested if applied to related species.

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