Anti-BRMS1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9HCU9

Core Product Specifications and Parameters

Biological Background: BRMS1 Function and Localization

• BRMS1 (Breast cancer metastasis-suppressor 1) is a transcriptional repressor that inhibits NF-kappa-B-mediated transcription by promoting deacetylation of RELA at Lys-310 and facilitating HDAC1 recruitment to target gene promoters.
• It suppresses the expression of anti-apoptotic genes regulated by NF-kappa-B, thereby promoting programmed cell death.
• BRMS1 induces anoikis, a form of apoptosis triggered by inadequate cell-substrate adhesion, which is a critical mechanism for preventing metastasis.
• The protein acts as a metastasis suppressor, particularly in breast carcinoma, by inhibiting the spread of cancer cells.
• Subcellularly, BRMS1 localizes to both the nucleus and cytoplasm, consistent with its roles in transcriptional regulation and signaling.
• Expression levels of BRMS1 are higher in term placentas compared to early placentas, with low expression observed in normal and molar pregnancies.
• Key structural features include coiled-coil domains, and the protein undergoes isopeptide bond formation and ubiquitination. It is classified as a tumor suppressor involved in transcription regulation and apoptosis.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 90-210 of human BRMS1, a region that may avoid homology with closely related proteins, but cross-reactivity with endogenous BRMS1 should be verified in knockout cell lines or tissues.
• For Western blot, a band at ~28 kDa is expected. Since BRMS1 may have low abundance, consider enriching nuclear extracts or using a positive control like breast cancer cell lines with known expression levels.
• In immunohistochemistry (IHC-P), antigen retrieval methods and antibody dilution should be optimized for each tissue type; validate with adjacent sections using a negative control (e.g., IgG isotype).
• For IF/ICC, both nuclear and cytoplasmic staining may be observed; include a nuclear counterstain to assess localization.
• ELISA performance relies on the recombinant immunogen; for sandwich ELISA, pairing with a detection antibody may be required. Validate with recombinant BRMS1 standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies high-quality raw materials, including this anti-BRMS1 antibody, to support research in cancer metastasis and apoptosis. Whether you need validated antibody pairs for immunoassay development, optimized monoclonal or polyclonal antibodies, or ancillary bulk reagents, our one-stop platform streamlines sourcing from concept to clinic. Contact us to discuss your specific requirements for rare target raw materials.