Anti-BRK1 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q8WUW1

Core Product Specifications and Parameters

Biological Background: BRK1 Function and Localization

• BRK1 (Protein BRICK1), also known as HSPC300, hHBrk1, MDS027, and C3orf10, is a small human protein encoded by the BRK1 gene.
• It plays a key role in regulating actin and microtubule organization by serving as a component of the WAVE complex, which directly activates the Arp2/3 complex to nucleate actin filament branching.
• As part of the WAVE1 complex, BRK1 is required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes, as inferred from similarity-based evidence.
• The protein localizes predominantly to the cytoplasm and is associated with the cytoskeleton, consistent with its role in actin dynamics.
• Post-translational modifications include acetylation, and structural features such as a coiled-coil domain have been identified; alternative splicing gives rise to multiple isoforms.
• The calculated molecular weight of the canonical isoform is approximately 8.7 kDa, aligning with the observed band around 9 kDa in experimental settings.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-75 of human BRK1 (NP_060932.2), covering the N-terminal region; this sequence is likely conserved in mouse, supporting the reported cross-reactivity.
• For IHC-P, consider optimizing antigen retrieval conditions and testing a range of antibody dilutions on relevant human or mouse tissue sections, using appropriate positive and negative controls.
• For IF/ICC, validate subcellular localization in cultured cells under fixation and permeabilization protocols suitable for cytoskeletal targets, and confirm specificity with knockdown or knockout controls if available.
• In ELISA, the recombinant protein used as immunogen can serve as a positive control to assess binding affinity and sensitivity; pairing with a detection antibody may aid assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop access to high-quality IVD raw materials, including validated antibodies for cytoskeletal and signaling targets like BRK1. Our portfolio spans monoclonal/polyclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and rare target sourcing, supporting every stage from concept to clinic. For BRK1-related projects, we offer flexible supply solutions tailored to your immunoassay development needs.