Anti-BRIX1 Polyclonal Antibody for WB, IHC-P, ELISA - Q8TDN6

Core Product Specifications and Parameters

Biological Background: BRIX1 Function and Localization

• BRIX1, also known as Brix domain-containing protein 2, is a ribosome biogenesis protein. It is a member of the Brix domain family, characterized by a conserved domain involved in ribosome assembly.
• The primary function of BRIX1 is to facilitate the biogenesis of the 60S ribosomal subunit, a critical step in ribosome maturation.
• Subcellularly, BRIX1 localizes to the nucleus, with enrichment in the nucleolus, the site of ribosomal RNA synthesis and ribosome subunit assembly.
• Post-translational modifications of BRIX1 include acetylation and phosphorylation, which may regulate its activity or interactions. It is also subject to Ubl conjugation, forming isopeptide bonds.
• High-throughput proteomics studies have identified BRIX1 in various cellular contexts, underscoring its ubiquitous role in ribosome biogenesis.
• The molecular weight of BRIX1 is approximately 41 kDa.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans the full-length human BRIX1 protein (amino acids 1-353). Consider this when predicting epitope accessibility in different applications.
• For Western blot analysis, validate the antibody in your specific sample system (e.g., HeLa, HEK293, or mouse/rat tissue lysates) and optimize protein loading to detect the ~41 kDa band.
• In immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) may need optimization for your tissue type.
• For ELISA, use the recombinant protein as a positive control and perform a titration to determine the optimal antibody dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw-material sourcing, from concept to clinic. We supply validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare targets like ribosome biogenesis factors. This anti-BRIX1 polyclonal antibody supports research in ribosome biology and nucleolar function, and CamelBio ensures reliable access to the raw materials needed for your diagnostic development.