Anti-BRIP1 Rabbit Polyclonal Antibody for WB and ELISA - Q9BX63

Core Product Specifications and Parameters

Biological Background: BRIP1 Function and Localization

• BRIP1 encodes Fanconi anemia group J protein (FANCJ), also known as BRCA1-associated C-terminal helicase 1 (BACH1) and BRCA1-interacting protein 1. It belongs to the DEAH helicase family and contains a 4Fe-4S iron-sulfur cluster domain critical for its helicase activity.
• Functions as a DNA-dependent ATPase and 5'-3' DNA helicase that maintains chromosomal stability by resolving DNA secondary structures during replication and repair. Related references: PMID:11301010 PMID:14983014 PMID:16116421
• Plays a key role in the Fanconi anemia (FA) pathway, acting downstream of FANCD2 ubiquitination to repair DNA interstrand crosslinks. Facilitates homologous recombination repair of DNA double-strand breaks through its BRCA1 interaction. Related references: PMID:14983014 PMID:16153896
• Removes abasic site–associated DNA-protein crosslinks at replication forks by helicase-driven unfolding of HMCES adducts, enabling SPRTN-mediated cleavage and lesion resolution. Related references: PMID:16116421 PMID:36608669
• Unwinds RNA:DNA hybrids and G-quadruplex (G4) DNA structures; G4 unwinding requires a 5′ single-stranded DNA tail, indicating specificity for replication-associated substrates. Related references: PMID:14983014 PMID:18426915 PMID:20639400
• Localizes to both the nucleus and cytoplasm, with ubiquitous expression and highest levels in testis. Undergoes post-translational modifications including phosphorylation and acetylation, and mutations in BRIP1 are linked to Fanconi anemia and cancer predisposition.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 260 amino acids of BRIP1, which may recognize full-length and potential N-terminal isoforms. Verify specificity using appropriate controls or knockdown/knockout models.
• For Western blotting, BRIP1 has a predicted molecular weight of ~141 kDa. Optimize transfer conditions for high-molecular-weight proteins and load sufficient protein (e.g., 30–50 µg nuclear extract) due to typically low endogenous expression.
• In ELISA applications, use recombinant BRIP1 protein (e.g., immunogen fragment) as a positive coating antigen and validate linearity/dynamic range with serial dilutions.
• DNA-damaging agents (e.g., mitomycin C or cisplatin) may upregulate BRIP1 expression or alter its subcellular localization; consider pretreatment of cells to enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials for DNA repair and genomic stability targets like BRIP1. We offer validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to streamline your assay development. Whether you need consistent lot-to-lot performance or specialized technical consultation, CamelBio bridges your concept-to-clinic journey.