Anti-BRINP3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q76B58

Core Product Specifications and Parameters

Biological Background: BRINP3 Function and Localization

• BRINP3 (BMP/retinoic acid-inducible neural-specific protein 3), also known as FAM5C or DBCCR1-like protein 1, is a secreted and mitochondrial glycoprotein implicated in cell cycle regulation.
• It inhibits neuronal cell proliferation by negatively regulating cell cycle progression, suggesting a role in growth arrest.
• Overexpressed BRINP3 promotes proliferation, migration, and invasion of pituitary gonadotrope cells and may contribute to pituitary tumor development.
• The protein is localized to both the extracellular space (secreted) and mitochondria, indicating distinct functional compartments.
• Tissue expression is prominent in oral keratinocytes, endothelial cells, and aortic smooth muscle cells; it is downregulated in tongue squamous cell carcinoma.
• BRINP3 is overexpressed in gonadotropinomas compared to normal pituitary tissues, linking it to endocrine tumor biology.
• Alternative splicing generates multiple isoforms, and the protein contains a signal peptide and potential glycosylation sites.
• Keywords: Alternative splicing, Cell cycle, Glycoprotein, Growth arrest, Mitochondrion, Proteomics identification, Reference proteome, Secreted, Signal.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal sequence (aa 240-500) of human BRINP3; consider that the antibody may recognize multiple splice isoforms if the epitope is conserved.
• For Western blot, use whole-cell lysates or mitochondrial-enriched fractions and validate the detected band near 88 kDa in relevant cell lines, as endogenous expression may vary.
• In immunofluorescence/ICC, include proper fixation and permeabilization steps to access mitochondrial pools; confirm staining specificity with negative controls.
• For ELISA, determine optimal coating conditions and antibody dilutions empirically, as performance can depend on target presentation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibodies like this anti-BRINP3 polyclonal antibody. Our portfolio supports neuroscience and oncology research, offering bulk ancillary reagents, custom antibody development, and rare target sourcing. As a comprehensive partner from concept to clinic, we help you accelerate assay development with reliable reagents and expert technical services.