Anti-BRG1/SMARCA4 Polyclonal Antibody for WB, IF/ICC, ELISA - P51532

Core Product Specifications and Parameters

Biological Background: BRG1/SMARCA4 Function and Localization

• SMARCA4 (BRG1) is the SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, also known as BAF190A, protein BRG-1, SNF2-beta, and transcription activator BRG1.
• Acts as an ATP-dependent chromatin remodeler, altering DNA-histone contacts to regulate transcription of select genes. Related references: PMID:15075294, PMID:29374058, PMID:30339381
• Core component of SWI/SNF chromatin remodeling complexes; in neural development, participates in the npBAF and nBAF complexes with subunit switching during differentiation.
• Functions as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for TGF-beta-induced epithelial-mesenchymal transition (EMT). Related references: PMID:20418909
• Localizes to the nucleus, where it associates with chromatin and exerts its remodeling function.
• Colocalizes with ZEB1 in E-cadherin-negative cells of established lines, stroma of normal colon, and at the invasion front of colorectal carcinomas.
• Subject to acetylation, phosphorylation, and ubiquitination; possesses bromodomain and ATPase domains, with RNA-binding activity.
• Keywords indicate involvement in deafness and intellectual disability (disease variants), and the protein undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 30-130) of human SMARCA4; this region is relatively conserved and may detect multiple isoforms or related family members.
• Given the large molecular weight (185 kDa), use an SDS-PAGE gel with 6–8% polyacrylamide for optimal separation in western blotting; a low voltage transfer for extended time is recommended.
• For immunofluorescence/ICC, consider nuclear permeabilization and a nuclear counterstain to confirm localization; enrichment of nuclear fractions can enhance signal.
• Validate antibody specificity in the relevant cell or tissue system, especially if cross-reactivity with other species is expected, as official cross-reactivity data is limited to human.
• ELISA performance may depend on the antigen conformation; a competitive or sandwich format using the immunogen peptide as standard can be considered.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials and technical services. This anti-BRG1/SMARCA4 polyclonal antibody is part of our extensive catalog of high-quality antibodies for chromatin biology and oncology research. Beyond individual reagents, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, enabling seamless integration from early-stage development to clinical applications.