Anti-BRG1/SMARCA4 Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P51532

Core Product Specifications and Parameters

Biological Background: BRG1/SMARCA4 Function and Localization

• BRG1/SMARCA4 (also known as BAF190A, SNF2-beta, and protein brahma homolog 1) is an ATP-dependent chromatin remodeler that is a core component of the SWI/SNF complex, altering DNA-nucleosome interactions to regulate transcription. Related references: PMID:15075294 PMID:29374058 PMID:30339381
• It functions as both a transcriptional activator and repressor, with crucial roles in neural development: it forms part of the npBAF and nBAF complexes, where subunit switching (e.g., ACTL6A/B, PHF10/DPF) drives the transition from proliferating neural progenitors to postmitotic neurons.
• BRG1/SMARCA4 mediates Notch-dependent proliferative signals in neural stem cells while suppressing SHH-dependent differentiation, thereby promoting self-renewal.
• In the CREST-BRG1 complex, it orchestrates calcium-dependent transcriptional activation by coordinating repressor release and activator recruitment at promoters such as c-FOS and NR2B.
• It acts as a corepressor for ZEB1, recruiting the machinery that represses E-cadherin transcription, a critical step in epithelial-mesenchymal transition (EMT). Related references: PMID:20418909
• Subcellular localization is predominantly nuclear, consistent with its chromatin-remodeling activity.
• Key structural features include an ATPase/helicase domain, a bromodomain for acetyl-lysine recognition, and multiple regulatory regions; it is subject to acetylation, phosphorylation, and ubiquitination, which modulate its activity.
• At the protein level, it colocalizes with ZEB1 in E-cadherin-negative cells of normal colon stroma and at the invasion front of colorectal carcinomas, suggesting a role in tumor invasion.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 200–300 of human BRG1/SMARCA4; this region may be conserved across species (Human, Mouse, Rat), supporting the observed cross-reactivity.
• For Western blot (WB), the predicted molecular weight is ~185 kDa; ensure adequate protein transfer and membrane blocking for high-molecular-weight proteins.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), the nuclear localization of BRG1/SMARCA4 should yield a specific nuclear signal; use appropriate fixation and permeabilization methods.
• For ELISA, the monoclonal nature of this antibody provides high specificity; consider pairing with capture/detection antibodies targeting non-overlapping epitopes.
• Validate antibody performance in your specific cell lines or tissue types, especially those relevant to EMT, neural development, or cancer research.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutions with high-quality IVD raw materials like this BRG1/SMARCA4 monoclonal antibody, essential for studying chromatin remodeling and transcriptional regulation. Our portfolio extends to validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to accelerate your assay development from concept to clinic.