Anti-BRF2 Rabbit Polyclonal Antibody for WB and ELISA - Q9HAW0

Core Product Specifications and Parameters

Biological Background: BRF2 Function and Localization

• BRF2 (B-related factor 2), also known as TFIIIB50 or hBRFU, is the 50 kDa subunit of transcription factor IIIB, a component of the RNA polymerase III transcription machinery.
• It functions as a general activator of RNA polymerase III transcription, specifically required for transcription of genes with promoter elements upstream of initiation sites. Related references: PMID:11040218, PMID:11121026, PMID:11564744
• BRF2 contributes to the regulation of gene expression in response to oxidative stress: it acts as an activator under normal conditions, but down-regulates target genes upon oxidative stress; overexpression protects against apoptosis. Related references: PMID:26638071
• The protein localizes to the nucleus, consistent with its role in transcription.
• BRF2 contains zinc-finger motifs and is subject to post-translational modifications such as phosphorylation and oxidation.
• Alternative splicing of the BRF2 gene produces multiple transcript variants, potentially yielding distinct isoforms.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein spanning amino acids 180-419 of human BRF2, targets a central region that is likely conserved across species and may detect multiple isoforms if they share this sequence.
• For Western blotting, consider using whole-cell lysates from human, mouse, or rat samples; expect a band around 47 kDa and validate with appropriate positive and negative controls.
• In ELISA applications, optimize coating concentrations and consider direct or sandwich formats based on detection requirements; cross-reactivity with mouse and rat samples may facilitate multispecies studies.
• Given BRF2’s role in oxidative stress response, evaluate expression changes under induced stress conditions (e.g., H2O2 treatment) to confirm functional relevance in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline procurement of high-quality IVD raw materials. This anti-BRF2 polyclonal antibody supports investigations into RNA polymerase III transcription and oxidative stress pathways. Beyond catalog antibodies, CamelBio can source or custom-develop critical reagents—including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents—tailored to your assay development needs.