Anti-BRD9 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H8M2

Core Product Specifications and Parameters

Biological Background: BRD9 Function and Localization

• BRD9 is a bromodomain-containing protein that reads acylated histone marks, binding to both acetylated and butyrylated histones, which implicates it in chromatin signaling (PubMed:26365797). Related references: PMID:26365797
• It is a key component of the GBAF SWI/SNF subcomplex, where it facilitates ATP-dependent nucleosome remodeling to regulate transcription (PubMed:29374058). Related references: PMID:29374058
• BRD9 contributes to transcriptional regulation via its role in chromatin remodeling, as demonstrated by functional studies (PubMed:22464331). Related references: PMID:22464331
• In the context of DNA repair, BRD9 promotes homologous recombination by orchestrating the assembly of the RAD51-RAD54 complex (PubMed:32457312). Related references: PMID:32457312
• BRD9 is exclusively nuclear, consistent with its functions in genome organization and expression.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, suggesting regulatory control over its activity.
• Initially identified as a rhabdomyosarcoma antigen, BRD9 may have implications in oncogenesis.

Experimental Guidance and Technical Tips

• This antibody is raised against a recombinant protein spanning amino acids 274-597 of human BRD9, covering the C-terminal region. It is expected to recognize the full-length protein (predicted ~67 kDa) in appropriate sample types.
• For Western blotting, a band corresponding to ~67 kDa may be observed. Use appropriate loading controls and consider the source species.
• In immunohistochemistry (paraffin-embedded sections), antigen retrieval conditions and antibody dilution may need optimization based on tissue type and fixation methods.
• Given the reported cross-reactivity with mouse and rat, this antibody can be used for conserved BRD9 detection in these species; however, independent validation in the target species is recommended.
• As a polyclonal antibody, lot-to-lot variability may occur, and it may exhibit multiple bands; confirm specificity with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibodies like this BRD9 polyclonal antibody for chromatin biology and oncology research. We offer bulk ancillary reagents and rare target sourcing to streamline your assay development from concept to clinic.