Anti-BRD2 Monoclonal Antibody for WB, ELISA - P25440

Core Product Specifications and Parameters

Biological Background: BRD2 Function and Localization

• BRD2, also known as RING3, is a bromodomain-containing protein that functions as a chromatin reader, specifically recognizing histone H4 acetylated at lysine 5 and lysine 12 (H4K5ac and H4K12ac), thereby modulating chromatin structure and gene expression. Related references: PMID:17148447 PMID:17848202 PMID:18406326

• It plays a critical role in genome compartmentalization by associating with CTCF and cohesin, promoting topologically associating domain (TAD) formation and contributing to boundary insulation and enhancer function. Related references: PMID:35410381

• BRD2 recruits transcription factors and coactivators to target gene promoters and activates RNA polymerase II for transcriptional elongation, partly through recognition of acetylated non-histone proteins such as STAT3. Related references: PMID:28262505

• In the immune system, BRD2 regulates inflammatory responses by directing naive CD4+ T-cell differentiation into Th17 cells; it binds acetylated STAT3 at lysine 87, facilitating STAT3 chromatin recruitment. Related references: PMID:28262505

• BRD2 also recognizes dual-modification marks on histones: N6-acetyl-N6-methyllysine (Kacme) on histone H4 at lysine 5 and lysine 12, an epigenetic signature of active chromatin linked to elevated transcriptional initiation. Related references: PMID:37731000

• BRD2 localizes predominantly to the nucleus and associates with chromosomes, consistent with its role in chromatin organization and transcriptional regulation.

• The protein contains two bromodomains and an ET domain; it undergoes alternative splicing, and post-translational modifications include acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The predicted molecular weight of BRD2 is approximately 88 kDa; a band near this size should be observed in Western blot analyses using appropriate human cell or tissue lysates.
• The immunogen corresponds to the N-terminal region (amino acids 1–345) of human BRD2; note that alternative splicing may produce isoforms lacking this region—confirm specificity in your sample system.
• For ELISA applications, consider using this antibody as a capture or detection reagent after thorough titration and cross-reactivity testing with relevant biological matrices.
• Cross-reactivity has only been validated for human BRD2; performance with samples from other species has not been tested and is not guaranteed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide a comprehensive portfolio of IVD raw materials and technical support, covering the entire workflow from concept to clinic. This anti-BRD2 monoclonal antibody, targeting a key chromatin reader and epigenetic regulator, is part of CamelBio’s curated collection of validated antibodies, matched pairs, and ancillary reagents tailored for immunoassay development and biomarker research. Whether you need bulk monoclonal antibodies, optimized detection pairs, or rare-target sourcing, our dedicated team streamlines the procurement process to accelerate your assay development and clinical translation.