Anti-BRCA2 Rabbit Polyclonal Antibody for WB - P51587

Core Product Specifications and Parameters

Biological Background: BRCA2 Function and Localization

• BRCA2 (Breast cancer type 2 susceptibility protein) is a tumor suppressor that maintains genome stability primarily by repairing DNA double-strand breaks through homologous recombination (HR).
  Related references: PMID:11239456, PMID:12442171, PMID:15115758

• It facilitates HR by binding and loading RAD51 recombinase onto single-stranded DNA (ssDNA), thereby promoting DNA strand exchange, a critical step in DNA repair.
  Related references: PMID:11239456, PMID:12442171, PMID:15937124

• Independently of HR, BRCA2 suppresses R-loop formation to prevent transcription-associated genomic instability and DNA damage.
  Related references: PMID:24896180

• BRCA2 promotes RAD51 loading to telomeric regions, facilitating telomere replication and capping, which is essential for chromosome integrity.
  Related references: PMID:21076401

• During meiosis, BRCA2 recruits RAD51 and DMC1 recombinases to meiotic double-strand breaks, enabling proper chromosome pairing and crossing over.
  Related references: PMID:26976601

• BRCA2 localizes to the nucleus, chromosomes, telomeres, and the centrosome, reflecting its multifaceted roles in DNA repair and cell division.

• Tissue expression is highest in breast and thymus, with lower levels in lung, ovary, and spleen, consistent with its tumor suppressor function in these tissues.

• Post-translational modifications include phosphorylation and ubiquitination, which regulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 1400-1600) lies within the BRC repeat domain of BRCA2; consider its potential to detect full-length protein and certain splice variants.
• For WB analysis, the high molecular weight (~384kDa) may require optimized transfer conditions, such as using 4-12% gradient gels, low methanol transfer buffer, and extended transfer times.
• The antibody has been shown to react with human, mouse, and rat BRCA2; validate cross-reactivity in your specific model system using appropriate controls.
• When performing IHC-P or IF/ICC, antigen retrieval and permeabilization steps may be essential; optimize based on sample type and fixation method.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, labs, and research institutes, offering one-stop access to IVD raw materials, technical services, and expert consulting. For BRCA2 research, we supply validated antibody pairs, optimized polyclonal antibodies like this BRCA2 Rabbit pAb, and bulk ancillary reagents to support DNA repair and cancer biology studies. Whether you need rare-target antibody development, large-scale production, or regulatory guidance, CamelBio bridges the gap from concept to clinic.