Anti-BRCA1 Polyclonal Antibody for WB and ELISA - P38398

Core Product Specifications and Parameters

Biological Background: BRCA1 Function and Localization

• BRCA1 is a 208 kDa nuclear phosphoprotein that acts as an E3 ubiquitin-protein ligase, specifically mediating 'Lys-6'-linked polyubiquitin chain formation. Related references: PMID:10500182, PMID:12887909, PMID:12890688
• As part of the BRCA1-BARD1 heterodimer, it plays a central role in DNA damage repair, coordinating homologous recombination, cell cycle checkpoints, and transcriptional regulation to maintain genomic stability. Related references: PMID:12890688, PMID:14976165, PMID:20351172
• Required for appropriate cell cycle arrests after ionizing radiation in both S-phase and G2 phase, and for FANCD2 targeting to DNA damage sites. Related references: PMID:10724175, PMID:11836499, PMID:12183412, PMID:12887909
• Contributes to homologous recombination repair via direct interaction with PALB2, facilitating loading of BRCA2-RAD51 repair machinery at DNA breaks. Related references: PMID:19369211
• Also regulates centrosomal microtubule nucleation, inhibits lipid synthesis by binding to inactive phosphorylated ACACA, and acts as a transcriptional activator. Related references: PMID:18056443, PMID:16326698, PMID:20160719
• Widely expressed as isoforms 1 and 3; isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
• Post-translationally modified by phosphorylation, acetylation, and ubiquitination; contains a RING-type zinc finger and is classified as a tumor suppressor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an N-terminal region (aa 100–200) of human BRCA1. The polyclonal nature may yield multiple epitope recognition; consider pre-adsorption if high specificity is required.
• For Western blot, expect a major band around 208 kDa. Optimize gel percentage (e.g., 6–8%) and transfer conditions for high molecular weight proteins.
• For ELISA, the antibody can be used for capture or detection; optimal dilution and pairing must be determined empirically.
• Cross-reactivity with mouse BRCA1 has been observed; validate performance if used in mouse model systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, supporting diagnostic manufacturers from early research through clinical application. For BRCA1-related assay development, we offer validated antibody pairs, recombinant proteins, and bulk ancillary reagents to streamline your workflow. Our global sourcing network can also secure rare or custom raw materials tailored to your unique diagnostic needs.