Anti-BRAP (IMP/BRAP2/RNF52) Polyclonal Antibody for WB, IHC-P, ELISA - Q7Z569

Core Product Specifications and Parameters

Biological Background: BRAP Function and Localization

• BRAP (BRCA1-associated protein), also known as IMP, BRAP2, or RNF52, is a Ras-responsive E3 ubiquitin ligase and a negative regulator of the MAP kinase signaling cascade.
• It functions by inhibiting the formation of Raf/MEK complexes, likely through inactivation of the KSR1 scaffold protein, thereby impeding mitogenic signal propagation.
• Upon Ras activation, BRAP undergoes auto-polyubiquitination, which releases its inhibition of Raf/MEK complex formation, linking E3 ligase activity to signal transduction.
• The protein contains a RING-type zinc finger and a coiled-coil domain, characteristic of its transferase and metal-binding properties; it is also a phosphoprotein.
• BRAP localizes to the cytoplasm, where it may also serve as a cytoplasmic retention factor, potentially regulating nuclear transport of signaling components.
• It is expressed in breast epithelial cell lines; the gene undergoes alternative splicing.
• Functional keywords: Ubl conjugation pathway, zinc-finger, metal-binding, reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–200) of human BRAP; this design may recognize full-length protein and potentially some splice isoforms in relevant sample systems.
• For Western blot, a band of approximately 67 kDa is expected; validate detection using appropriate positive control lysates (e.g., human, mouse, or rat cell lines expressing BRAP).
• When performing IHC-P, consider using standard antigen retrieval methods and primary antibody titration to optimize staining in tissues of interest.
• ELISA can be employed for quantitative BRAP analysis; cross-reactivity to mouse and rat suggests utility in comparative studies.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical applications; including internal reference samples is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-BRAP polyclonal, to support research and assay development in Ras-MAPK signaling and cancer biomarker programs. Our supply capabilities extend to antibody pairs, ancillary reagents, and customized sourcing for rare targets, helping diagnostic manufacturers and academic laboratories streamline their workflow from concept to clinic. Contact us to explore bulk and kit-compatible formats tailored to your needs.