Anti-BRAF V600E Monoclonal Antibody for WB - P15056

Core Product Specifications and Parameters

Biological Background: BRAF Function and Localization

• BRAF (proto-oncogene B-Raf) is a serine/threonine-protein kinase that transduces mitogenic signals from the cell membrane to the nucleus.
• It phosphorylates MAP2K1, thereby activating the MAP kinase signal transduction pathway. Related references: PMID:21441910 / PMID:29433126
• It phosphorylates PFKFB2, contributing to metabolic regulation. Related references: PMID:36402789
• BRAF may play a role in the postsynaptic responses of hippocampal neurons. Related references: PMID:1508179
• It is located in the nucleus, cytoplasm, and cell membrane.
• BRAF is predominantly expressed in brain and testis.
• The protein undergoes extensive regulatory modifications, including phosphorylation, acetylation, methylation, and ubiquitination, and contains a zinc-finger domain. The V600E mutation leads to constitutive kinase activity and is a major oncogenic driver.

Experimental Guidance and Technical Tips

• The immunogen peptide spans amino acids 507–606, covering the V600 codon; therefore, this antibody is designed to detect the BRAF V600E mutant.
• For western blotting, the expected molecular weight is ~84 kDa. Validate using human cell lines known to express the V600E mutation, alongside wild-type controls.
• For immunofluorescence and immunocytochemistry, optimize fixation and permeabilization conditions according to the cellular context.
• When performing ELISA, consider coating with the immunizing peptide or recombinant mutant BRAF protein to assess antibody specificity.
• Cross-reactivity is reported for human; reactivity with other species should be tested individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. This anti-BRAF V600E monoclonal antibody is offered alongside our portfolio of validated antibody pairs, optimized antibodies, bulk ancillary reagents, and specialized raw-material sourcing for rare targets. Whether advancing oncological diagnostics or probing the MAPK pathway, CamelBio helps streamline your supply chain with reliable, high-quality materials.