Anti-Brachyury Polyclonal Antibody for WB, IF/ICC, ELISA - O15178

Core Product Specifications and Parameters

Biological Background: Brachyury Function and Localization

• Encoded by the TBXT gene, Brachyury (also known as T-box transcription factor T or Protein T) is a highly conserved developmental transcription factor.
• Plays a critical role in mesoderm formation and differentiation by regulating transcription of targeted genes.
• Recognizes and binds to a palindromic T-site DNA consensus sequence (5'-TTCACACCTAGGTGTGAA-3'), thereby activating gene expression.
• Localizes predominantly to the nucleus, consistent with its function as a DNA-binding transcriptional activator.
• Tissue expression is restricted; detected in testis and aberrantly expressed in lung tumors at the protein level.
• Classified under key UniProt keywords including developmental protein, activator, alternative splicing, and disease variant, highlighting its importance in both normal development and pathologies.

Experimental Guidance and Technical Tips

• The immunogen is designed against amino acids 226–435 of human Brachyury; this region includes the DNA-binding domain, which may recognize both full-length and splice variants.
• In Western blot (WB), a band around 47 kDa is expected; always include positive and negative controls to verify specificity.
• For immunofluorescence/ICC, anticipate nuclear staining; validate in cell lines known to express Brachyury (e.g., certain cancer cell lines).
• When developing ELISA, consider pairing with a capture antibody targeting a distinct epitope and titrate concentrations to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers by offering a seamless supply channel for IVD raw materials. In addition to this anti-Brachyury antibody for developmental and oncology studies, we provide validated antibody pairs, bulk polyclonal/monoclonal antibodies, and ancillary reagents to accelerate your assay development. Trust CamelBio to source even rare-target raw materials, ensuring you stay on track from concept to clinic.