Anti-BOULE/BOLL Monoclonal Antibody for WB, IP, IF-P, IHC-P, ELISA - Q8N9W6

Core Product Specifications and Parameters

Biological Background: BOLL Function and Localization

• Protein boule-like (BOLL) is an RNA-binding protein encoded by the BOLL gene in humans, also known as BOULE, with a molecular weight of approximately 31 kDa.
• It contains a putative RNA recognition motif, suggesting a role in binding to the 3'-untranslated region (UTR) of target mRNAs to regulate their translation.
• BOLL is a member of the DAZ (Deleted in Azoospermia) gene family, closely related to DAZ and DAZL, which are critical for germ cell development.
• Its expression is testis-specific, first detectable in the cytoplasm of spermatocytes and maintained throughout meiosis, indicating a role in spermatogenesis.
• BOLL is localized predominantly in the cytoplasm, consistent with its role in post-transcriptional regulation of maternal and early embryonic transcripts.
• Alternative splicing of the BOLL transcript generates multiple isoforms, which may contribute to functional diversity in spermatogenesis.
• As a developmental protein, BOLL is involved in the differentiation of male germ cells, bridging mitotic expansion and meiotic entry.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody covers the N-terminal region (amino acids 1–150) of human BOLL. For species such as mouse and rat, verify sequence homology in this region, as cross-reactivity is expected based on supplied data.
• For Western blotting, the predicted molecular weight is approximately 31 kDa; consider loading appropriate positive controls such as human, mouse, or rat testis lysates where BOLL is specifically expressed.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF-P), ensure antigen retrieval methods are optimized for testis tissue sections, as BOLL is exclusively expressed in spermatocytes and meiotic germ cells.
• When using for immunoprecipitation (IP) or ELISA, validate the antibody’s ability to capture native protein under non-denaturing conditions, given its recognition of an N-terminal epitope.
• Since BOLL is an RNA-binding protein with potential post-translational modifications, run reducing and non-reducing SDS-PAGE to assess any size shifts due to modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines raw material procurement for diagnostic manufacturers and research laboratories by offering verified monoclonal antibodies like this anti-BOLL rabbit mAb, essential for reproductive health studies. Our integrated platform provides validated antibody pairs, custom bulk orders, and complementary reagents to accelerate assay development and research into infertility biomarkers.