Anti-BOLA3 Polyclonal Antibody for WB, IHC-P, ELISA - Q53S33

Core Product Specifications and Parameters

Biological Background: BOLA3 Function and Localization

• BOLA3 (BolA-like protein 3), also known as MMDS2, is a mitochondrial protein involved in iron-sulfur (Fe-S) cluster biogenesis.
• Acts as a mitochondrial Fe-S cluster assembly factor that facilitates Fe-S cluster insertion into a subset of mitochondrial proteins, probably in cooperation with NFU1. Related references: PMID:27532772
• Localizes to the mitochondrion, consistent with its role in mitochondrial Fe-S cluster assembly.
• Widely expressed across tissues, suggesting a fundamental housekeeping role in cellular metabolism.
• Mutations in BOLA3 are associated with primary mitochondrial disease, highlighting its clinical significance.
• Alternative splicing generates multiple transcript variants, potentially affecting protein function or localization.
• The protein has a molecular weight of approximately 12 kDa, and its three-dimensional structure has been determined, supporting functional studies.
• Identified through proteomics studies, confirming expression at the protein level.

Experimental Guidance and Technical Tips

• Due to the small molecular weight of BOLA3 (~12 kDa), use a 15% SDS-PAGE gel and optimize transfer conditions for Western blotting to ensure clear resolution and transfer.
• For immunohistochemistry (IHC-P), validate the antibody in relevant tissue samples, considering the widespread expression of BOLA3.
• In ELISA applications, test the antibody as a capture or detection reagent and optimize coating concentrations based on the specific assay format.
• As a polyclonal antibody, it may recognize multiple epitopes; consider performing lot-to-lot consistency testing for quantitative assays.
• Cross-reactivity with mouse and rat enables translational studies in these model organisms; confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials. This anti-BOLA3 polyclonal antibody, part of our mitochondrial biology portfolio, supports assay development for iron-sulfur cluster assembly and mitochondrial disorder research. From validated antibody pairs to bulk ancillary reagents, we offer one-stop procurement solutions to accelerate your diagnostic projects.