Anti-BOK Rabbit Monoclonal Antibody for WB and ELISA - Q9UMX3

Core Product Specifications and Parameters

Biological Background: BOK Function and Localization

• Bcl-2-related ovarian killer protein (BOK) is a Bcl-2 family member and apoptosis regulator that can act as both a pro- and anti-apoptotic protein depending on cellular context.
• It promotes intrinsic apoptosis via mitochondrial outer membrane permeabilization, either dependently or independently of BAX/BAK1, in response to ER stress or proteasome inhibition. Related references: PMID:15102863 PMID:27076518
• BOK mediates TP53-dependent apoptosis following DNA damage. Related references: PMID:15102863
• It plays a role in granulosa cell apoptosis through CASP3 activation. Related references: PMID:20673843
• Beyond apoptosis, BOK can induce autophagy by disrupting the MCL1-BECN1 interaction. Related references: PMID:24113155
• BOK is expressed in multiple subcellular compartments including mitochondria, endoplasmic reticulum, Golgi, endosomes, nucleus, and cytoplasm.
• In adult human ovaries, BOK is strongest in primordial/primary follicle granulosa cells, consistent with a role in oocyte atresia.
• Additional keywords: 3D-structure available, alternative splicing, phosphoprotein, ubiquitin conjugation, transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human BOK; the antibody may recognize both full-length and truncated isoforms if the epitope is intact.
• For WB, consider using mitochondrial-enriched lysates from human, mouse, or rat cell lines to enhance detection of the ~23 kDa band.
• Validate antibody specificity in your experimental system using knockdown/knockout controls.
• The antibody has been tested for ELISA; for coating antigen, consider the immunogen peptide or recombinant BOK fragments.
• Optimize primary antibody dilution for your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibodies, antigen pairs, and ancillary reagents to accelerate assay development. For apoptosis and mitochondrial pathway research, our portfolio includes high-quality monoclonal/polyclonal antibodies against BCL2 family members like BOK, along with customized bulk supply and rare-target sourcing capabilities. Partner with us to streamline your diagnostic raw-material procurement from concept to clinic.