Anti-BOB1 Monoclonal Antibody for WB, IF/ICC, IHC-P, ELISA - Q16633

Core Product Specifications and Parameters

Biological Background: BOB1 Function and Localization

• Transcriptional coactivator that specifically associates with POU2F1/OCT1 or POU2F2/OCT2, enhancing their promoter activity. Related references: PMID:7859290, PMID:7779176
• Essential for B-cell antigen response and germinal center formation. Related references: PMID:7623806, PMID:7859290
• Regulates IL6 expression in B cells as a coactivator of POU2F2/OCT2 (by similarity).
• Localizes exclusively to the nucleus, consistent with its role as a transcription factor.
• B-cell specific expression, primarily detected in spleen, with lower levels in thymus, peripheral blood leukocytes, and small intestine. Related references: PMID:7779176, PMID:7859290
• Designated as a proto-oncogene; involved in chromosomal rearrangements and subject to ubiquitin-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 150-250 of human BOB1, suggesting the antibody may recognize epitopes within the central region of the protein.
• The antibody has been validated for Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA. Consider using appropriate positive controls such as human B-lymphocyte cell lines for functional assays.
• Due to the nuclear localization of BOB1, ensure proper permeabilization steps during IF/ICC and IHC-P protocols to allow antibody access to the nucleus.
• For WB, a band at approximately 27 kDa is expected; validate loading with a reference protein comparing B-cell versus non-B-cell lysates.
• The antibody cross-reacts with human, mouse, and rat samples; consider species-specific negative and positive controls to confirm specificity in non-human models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes streamlined access to high-quality IVD raw materials, including validated monoclonal antibodies like the Anti-BOB1 Rabbit mAb. Whether you require optimized antibody pairs, bulk ancillary reagents, or specialized sourcing for rare targets in B-cell biology and immuno-oncology, our one-stop platform bridges the gap from concept to clinical application with reliable supply and technical support.