Anti-BNP Rabbit Monoclonal Antibody for WB, ELISA - P16860

Core Product Specifications and Parameters

Biological Background: BNP Function and Localization

• BNP (Natriuretic peptides B, also known as brain natriuretic factor prohormone or Iso-ANP) is a cardiac hormone that mediates cardio-renal homeostasis by binding NPR1, stimulating cGMP production, and regulating fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. It also binds the clearance receptor NPR3. Related references: PMID:1672777, PMID:17372040, PMID:1914098, PMID:17349887, PMID:9458824, PMID:16870210

• Processed forms such as brain natriuretic peptide 32 exhibit low potency in cGMP production but may still contribute to cardio-renal homeostasis. Related references: PMID:17372040

• BNP is secreted and predominantly detected in cardiac atria and kidney distal tubular cells, with additional expression in bone growth plate chondrocytes (late proliferative to hypertrophic). Related references: PMID:2136732, PMID:2138890, PMID:9794555

• As a member of the natriuretic peptide family, BNP features a disulfide bond, glycosylation, and proteoglycan modifications, and acts as a vasodilator.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within the propeptide region (aa 35-134). This antibody may detect both proBNP and processed BNP peptides in WB. Due to the low molecular weight of mature BNP (~3.5 kDa), careful gel conditions and transfer settings are recommended.
• For ELISA applications, consider pairing with capture antibodies recognizing distinct epitopes for sandwich detection.
• Validate specificity in relevant human, mouse, or rat cardiac tissue or plasma samples, as cross-reactivity has been reported.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. For researchers studying cardiovascular disease and natriuretic peptide signaling, we provide dependable access to BNP antibodies and related assay components. Our one-stop sourcing bridge supports diagnostic manufacturers and labs from concept to clinic.