Anti-BNP Polyclonal Antibody for WB, IF, IHC, ELISA - P16860

Core Product Specifications and Parameters

Biological Background: BNP Function and Localization

• BNP (Brain Natriuretic Peptide) is initially synthesized as the preprohormone preproBNP, which is processed to proBNP and further cleaved into the biologically active 32-amino acid peptide (BNP-32) and the N-terminal fragment NT-proBNP.
• As a cardiac hormone, BNP specifically binds to the NPR1 receptor, stimulating cGMP production and triggering vasorelaxation and natriuretic responses (PubMed:1672777, PubMed:17349887, PubMed:17372040).
  Related references: PMID:1672777, PMID:17349887, PMID:17372040
• BNP plays a critical role in maintaining cardio-renal homeostasis by promoting diuresis, natriuresis, and inhibiting renin and aldosterone secretion (PubMed:1914098, PubMed:9458824).
  Related references: PMID:1914098, PMID:9458824
• The peptide also interacts with the clearance receptor NPR3, which modulates its circulating levels and bioactivity (PubMed:16870210).
  Related references: PMID:16870210
• BNP is secreted and primarily detected in cardiac atrial tissue, but expression is also found in the kidney distal tubules and in the hypertrophic zone of bone growth plate chondrocytes.
• Key post‑translational modifications include a disulfide bond and glycosylation; BNP belongs to the natriuretic peptide family and is classified as a vasodilator hormone with pharmaceutical relevance.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 35–134 of human BNP, encompassing the propeptide region that includes the mature BNP-32 sequence; thus, the polyclonal antibody may detect both the precursor (proBNP, ~15 kDa) and processed forms.
• For Western blot (WB), expect bands near 15 kDa for proBNP and possibly smaller bands corresponding to mature BNP or degradation products. Optimize sample preparation and reducing conditions to preserve epitopes.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate staining in positive control tissues such as human cardiac atria or kidney distal tubules. Antigen retrieval methods and antibody dilution should be empirically determined.
• For ELISA applications, consider pairing this polyclonal antibody with a matched monoclonal antibody to build a sensitive sandwich assay for BNP or NT-proBNP detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, connecting diagnostic manufacturers and research laboratories with rigorously validated antibody products, bulk reagents, and custom sourcing solutions. This anti-BNP polyclonal antibody supports cardiovascular biomarker development, heart failure diagnostics, and renal pathophysiology studies. For projects requiring rare or tailored raw materials, CamelBio’s integrated supply chain and technical expertise streamline the transition from assay concept to clinical application.