Anti-BNIP3L Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O60238

Core Product Specifications and Parameters

Biological Background: BNIP3L Function and Localization

• BNIP3L, also known as NIX, is a pro-apoptotic member of the BCL2 protein family that interacts with viral and cellular anti-apoptotic proteins, including BCL-2 and BCL-XL, to promote programmed cell death.
• It participates in mitochondrial quality control by mediating the degradation of damaged proteins inside mitochondria through interaction with SPATA18/MIEAP, a process called MALM (mitochondrial protein catabolic process).
• BNIP3L interacts with SPATA18/MIEAP at the mitochondrial outer membrane to regulate the opening of a pore in the mitochondrial double membrane, allowing translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix.
• The protein is localized to multiple membranes, including the mitochondrial outer membrane, nucleus envelope, and endoplasmic reticulum, reflecting its diverse roles in apoptosis and mitochondrial homeostasis.
• BNIP3L contains a transmembrane helix and undergoes alternative splicing, yielding isoforms that may differ in function or regulation.
• It may function as a tumor suppressor, linking its apoptotic activity to cancer biology.
• Key post-translational modifications include phosphorylation, and the protein is involved in host-virus interactions, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (amino acids 95-180) of human BNIP3L, which is conserved across species, supporting the observed cross-reactivity with mouse and rat.
• For Western blot (WB), use a starting dilution of 1:500-1:2000 and expect a band at approximately 24 kDa; optimize blocking and wash conditions to reduce background from polyclonal antisera.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval may be necessary due to the membrane localization of BNIP3L; validate in positive control tissues or cell lines known to express BNIP3L.
• For ELISA, use the recombinant immunogen as a positive control to establish the detection range.
• Consider co-immunoprecipitation experiments to study BNIP3L interactions with BCL-2 family members, given its known binding properties.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated IVD raw materials, including this BNIP3L rabbit polyclonal antibody for apoptosis and mitochondrial quality control studies. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, helping you accelerate assay development from concept to clinic.