Anti-BNIP3 Rabbit Polyclonal Antibody for IF/ICC and ELISA - Q12983

Core Product Specifications and Parameters

Biological Background: BNIP3 Function and Localization

• BNIP3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3), also known as NIP3, is a pro-apoptotic protein that can overcome BCL2 suppression.
• It participates in mitochondrial quality control by interacting with SPATA18/MIEAP, mediating the degradation of damaged mitochondrial proteins through the MALM pathway.
• BNIP3, together with BNIP3L/NIX, forms a pore at the mitochondrial outer membrane, facilitating the translocation of lysosomal proteins into the mitochondrial matrix for protein degradation.
• The protein plays a role in calprotectin (S100A8/A9)-induced cell death, linking inflammatory signals to mitochondrial dysfunction.
• Subcellular localization is predominantly at the mitochondrion outer membrane, consistent with its role in mitochondrial quality control and apoptosis.
• It undergoes phosphorylation and has a determined 3D structure; classified under keywords including apoptosis, host-virus interaction, and mitochondrial outer membrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-140 amino acids of human BNIP3, potentially targeting a functionally relevant region involved in protein-protein interactions.
• For immunofluorescence (IF/ICC), consider using mitochondrial markers for co-localization and optimize permeabilization conditions to access the outer membrane.
• ELISA applications may benefit from using recombinant BNIP3 protein or cell lysates from human cell lines known to express BNIP3, such as those undergoing hypoxia or apoptosis.
• Validate antibody specificity in your experimental system using appropriate controls, as cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with high-quality IVD raw materials like this BNIP3 rabbit polyclonal antibody, supporting apoptosis and mitochondrial research. As a comprehensive sourcing partner, we provide validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and rare-target sourcing to streamline your workflow from concept to clinic. Inquire about customized procurement solutions for your specific assay development needs.