Anti-BNIP2 Polyclonal Antibody for WB & ELISA - Q12982

Core Product Specifications and Parameters

Biological Background: BNIP2 Function and Localization

• BNIP2, also known as NIP2, is a BCL-2 and adenovirus E1B 19 kDa-interacting protein implicated in the suppression of cell death.
• It belongs to the BNIP family of proteins, characterized by their interaction with BCL-2 family members to regulate apoptosis.
• BNIP2 is predominantly localized to the cytoplasm, with enrichment in the perinuclear region.
• The protein undergoes phosphorylation and has multiple isoforms due to alternative splicing.
• It directly interacts with BCL-2 and the adenovirus E1B 19 kDa protein, both central regulators of apoptotic pathways.
• BNIP2 has been identified in proteomics screens and is part of the human reference proteome.

Experimental Guidance and Technical Tips

• The antibody is generated against a recombinant fragment corresponding to amino acids 125-280 of human BNIP2. For Western blot, expect a specific band at ~36 kDa; include appropriate positive and negative controls.
• For ELISA applications, titrate the antibody to determine the optimal working concentration and validate with recombinant BNIP2 protein or cell lysates.
• As a polyclonal antibody, it may recognize multiple epitopes; consider using a blocking agent to reduce non-specific binding.
• Cross-reactivity has been reported with mouse samples; validate in your own experimental system if working with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For apoptosis and cell death research, we supply this anti-BNIP2 polyclonal antibody as part of our commitment to bridging the gap between concept and clinic. From common to rare targets, our sourcing expertise ensures you have reliable materials for every stage of development.