Anti-BNIP1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q12981

Core Product Specifications and Parameters

Biological Background: BNIP1 Function and Localization

• BNIP1, also known as SEC20, TRG-8, or NIP1, is a vesicle transport protein and a member of the SNARE complex, playing a critical role in endoplasmic reticulum (ER) membrane fusion and maintenance of ER organization. Related references: PMID:15272311
• It is involved in apoptosis, mediating ER stress-induced cell death, and interacts with BCL2/adenovirus E1B 19 kDa protein. Related references: PMID:15272311, PMID:23896122, PMID:7954800
• As a substrate of RNF185 and interactor with SQSTM1, BNIP1 may also participate in mitochondrial autophagy.
• BNIP1 localizes to both the endoplasmic reticulum and mitochondrial membranes, consistent with its dual roles in vesicle transport and apoptosis.
• Tissue-specific expression: Isoform 1 is highly expressed in heart, brain, liver, skeletal muscle, and pancreas; isoform 3 is moderate in placenta, lung, and kidney; isoform 4 is high in testis and small intestine.
• Keywords associated with BNIP1 include alternative splicing, coiled coil domain, transmembrane helix, and Ubl conjugation, reflecting its structural and regulatory features.
• BNIP1 is associated with host-virus interaction processes, as indicated by keyword annotation.

Experimental Guidance and Technical Tips

• For Western blot, a band around 26 kDa is expected based on the predicted molecular weight of BNIP1; optimize sample preparation to preserve protein integrity.
• In immunohistochemistry, tissues with high BNIP1 expression (e.g., heart, brain) may yield optimal staining; include appropriate positive and negative controls.
• Cross-reactivity with mouse and rat has been reported; confirm specificity in your model system using species-appropriate secondary antibodies and lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research institutes with seamless access to this anti-BNIP1 monoclonal antibody, alongside a full portfolio of IVD raw materials including validated antibody pairs, blocking buffers, and ancillary reagents for ER stress and apoptosis studies. Whether you need bulk supplies or support sourcing rare targets, our integrated procurement platform and technical consulting streamline your workflow from concept to clinic.