Anti-BMX Polyclonal Antibody for WB, ELISA - P51813

Core Product Specifications and Parameters

Biological Background: BMX Function and Localization

• BMX is a non-receptor tyrosine kinase that plays central modulatory roles in actin reorganization, cell migration, proliferation, survival, adhesion, and apoptosis.
• It participates in signal transduction downstream of growth factor receptors, cytokine receptors, G protein-coupled receptors, antigen receptors, and integrins.
• BMX mediates integrin signaling by inducing tyrosine phosphorylation of BCAR1, and its activation by integrins requires PTK2/FAK1.
• It is critical for TNF-induced angiogenesis and is implicated in TEK and FLT1 receptor signaling, essential for angiogenesis.
• BMX is required for STAT3 phosphorylation and activation, contributing to cell differentiation; it is also involved in IL6-induced differentiation.
• The kinase plays a role in adaptive cytoprotection against extracellular stress in various cell types, including salivary epithelial, brain endothelial, and dermal fibroblasts.
• BMX may regulate endocytosis via interaction with RUFY1 and has roles in hematopoietic cell growth/differentiation and endothelial cell signal transduction.
• It is localized to the cytoplasm and is highly expressed in cells with high migratory potential, such as endothelial cells and metastatic carcinoma cell lines.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-120) of human BMX; consider epitope conservation when used with mouse samples.
• For WB, use whole-cell lysates from human or mouse endothelial or hematopoietic cell lines; the predicted molecular weight is 78kDa.
• Validate antibody specificity with appropriate positive (e.g., endothelial cell lysates) and negative controls (e.g., BMX knockdown or knockout samples).
• For ELISA, standard indirect or sandwich protocols may be employed; perform titration to determine optimal antibody concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing platform for IVD raw materials, including validated antibodies such as this anti-BMX polyclonal antibody. Our portfolio covers optimized antibody pairs, bulk ancillary reagents, and rare-target raw-material procurement, supporting your projects in signal transduction, angiogenesis, and oncology research from concept to clinic.