Anti-BMPR2 Rabbit Polyclonal Antibody - Q13873

Core Product Specifications and Parameters

Biological Background: BMPR2 Function and Localization

• BMPR2 (Bone morphogenetic protein receptor type-2, BMPR-II) is a transmembrane serine/threonine kinase receptor, with a molecular weight of ~115 kDa, and belongs to the transforming growth factor beta (TGF-β) receptor superfamily.
• Upon ligand binding (e.g., BMP2, BMP7), BMPR2 forms a heterotetrameric complex with type I receptors, leading to phosphorylation and activation of the type I receptor, which subsequently phosphorylates SMAD transcription factors (SMAD1/5/8) to regulate gene expression.
• BMPR2 can also mediate non-canonical signaling through the p38MAPK cascade, as demonstrated in certain cellular contexts.
  Related references: PMID:12045205
• BMPR2 binds BMP7 and BMP2 with high affinity, BMP4 less efficiently, and this binding is enhanced by the presence of type I receptors. Additionally, it can signal via activin A/INHBA.
  Related references: PMID:24018044
• The receptor is localized primarily at the cell membrane.
• It is highly expressed in heart and liver tissues.
• BMPR2 is subject to post-translational modifications including glycosylation and phosphorylation, as indicated by keywords: Glycoprotein, Phosphoprotein.
• Mutations in BMPR2 are associated with pulmonary arterial hypertension (PAH), reflecting its role as a disease variant biomarker.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 27-150) of human BMPR2, suggesting the antibody likely recognizes the extracellular region. This may be suitable for detecting both full-length and soluble receptor forms.
• For Western blot (WB), the expected band size is ~115 kDa for the full-length receptor. Consider using appropriate molecular weight markers and validate with relevant positive control cell lysates (e.g., heart or liver tissue).
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), BMPR2 is localized to the cell membrane; ensure proper permeabilization and include membrane marker co-staining for specificity validation.
• Cross-reactivity is confirmed with human, mouse, and rat species. For experiments using other species, sequence homology alignment of the immunogen region is recommended.
• For ELISA applications, the antibody can be used as a capture or detection reagent; optimize coating concentrations and validate with recombinant BMPR2 protein standards.
• Since BMPR2 signaling involves SMAD and p38MAPK pathways, functional studies may benefit from combining this antibody with phospho-specific antibodies for downstream signaling analysis.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a comprehensive, one-stop sourcing solution for IVD raw materials, including a broad portfolio of validated antibodies, recombinant antigens, and ancillary reagents. This anti-BMPR2 antibody supports research into cardiovascular biology and TGF-β signaling pathways. Beyond catalog products, CamelBio can assist with custom antibody development, rare target sourcing, and bulk supply to help accelerate your assay development from concept to clinic.