Anti-BMP7 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P18075

Core Product Specifications and Parameters

Biological Background: BMP7 Function and Localization

• BMP7 (bone morphogenetic protein 7), also known as osteogenic protein 1 (OP-1), is a secreted growth factor belonging to the TGF-beta superfamily, with key roles in embryogenesis, hematopoiesis, neurogenesis, and skeletal morphogenesis. Related references: PMID:31208997
• Canonical signaling is initiated when BMP7 binds to type I receptor ACVR1 and type II receptor ACVR2A, leading to phosphorylation of SMAD1/5/8 transcription factors that regulate target gene expression. Related references: PMID:12667445, PMID:9748228, PMID:12478285
• In specific processes like growth cone collapse and monocyte chemotaxis, BMP7 utilizes the alternative type II receptor BMPR2. Related references: PMID:31208997
• Non-canonical P38 MAP kinase signaling can be activated to drive brown adipocyte differentiation through SOX family transcription factors. Related references: PMID:27923061
• BMP7 promotes expression of the hepcidin gene HAMP; this induction is counteracted by the erythroid factor ERFE. Related references: PMID:30097509
• The protein is significantly expressed in kidney and bladder, with lower detection in brain. It contains a signal peptide, disulfide bonds, and N-glycosylation sites, consistent with its secretory nature.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 70–251 of the BMP7 proprotein; therefore, the antibody may recognize both full-length pro-BMP7 and mature BMP7 forms after proteolytic processing. Consider assessing cleavage patterns in your experimental system.
• For Western blot analysis, use reducing conditions and load positive control lysates from kidney or bone tissue to observe a band near 49 kDa; additional bands from precursor or degradation products may be present.
• In IHC-P, antigen retrieval with citrate buffer (pH 6.0) is recommended, and staining should be validated using tissues known to express BMP7, such as kidney.
• For ELISA, the polyclonal antibody can serve as a capture or detection reagent; pairing with a specific monoclonal antibody may enhance sensitivity and reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement platform for IVD raw materials, from validated antibody pairs to bulk ancillary reagents and specialized rare-target sourcing. Our BMP7 polyclonal antibody—ideal for investigations into TGF-beta signaling, bone morphogenesis, and developmental biology—exemplifies our commitment to providing reliable, high-quality immunoreagents for diagnostic and research applications. Contact CamelBio to explore how our one-stop supply chain solutions can accelerate your assay development from concept to clinic.