Anti-BMP6 Polyclonal Antibody for WB and ELISA - P22004

Core Product Specifications and Parameters

Biological Background: BMP6 Function and Localization

• BMP6 is a member of the TGF-beta superfamily, functioning as a secreted growth factor essential for developmental processes such as cartilage and bone formation. Related references: PMID:31019025
• It plays a critical role in iron homeostasis by regulating the expression of HAMP/hepcidin through interactions with hemojuvelin (HJV) and the BMP receptor BMPR1A/ALK3. Related references: PMID:26582087, PMID:30097509, PMID:31800957
• BMP6 initiates canonical signaling by binding to type I receptor ACVR1 and type II receptor ACVR2B, leading to phosphorylation of SMAD1/5/8 transcription factors. Related references: PMID:18070108
• It can also signal through non-canonical pathways, including the TAZ-Hippo cascade, to modulate VEGF signaling by regulating VEGFR2 expression. Related references: PMID:33021694
• The protein is secreted and undergoes post-translational modifications such as cleavage on basic residues, disulfide bond formation, and glycosylation (Keywords: Cleavage on pair of basic residues, Disulfide bond, Glycoprotein).
• Associated keywords include 3D-structure, Chondrogenesis, Cytokine, Developmental protein, Differentiation, Disease variant, Growth factor, Osteogenesis, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 375-513) of human BMP6; the antibody may recognize both the precursor and mature cleaved forms. Validate the detected band size in your sample system.
• For Western blot, consider using reducing conditions and testing a range of protein inputs to confirm specificity. A band near 57 kDa (precursor) or smaller (mature) may be observed.
• BMP6 is a secreted protein; conditioned medium or cell lysates from relevant cell lines (e.g., osteoblasts, hepatocytes) can be used for detection.
• For ELISA applications, optimize coating antigen and antibody pairing; cross-reactivity with mouse and rat BMP6 suggests conserved epitopes suitable for cross-species assays.
• As a polyclonal antibody, each lot may exhibit slight variation; always perform appropriate controls and titrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a reliable one-stop platform for IVD raw materials, including validated antibody pairs, optimally performing monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. The BMP6 polyclonal antibody supports investigations into bone development, iron metabolism, and TGF-beta signaling pathways. Partner with CamelBio to bridge your product development from concept to clinic with seamless supply and technical expertise.