Anti-BMP6 Polyclonal Antibody for IF/ICC and ELISA - P22004

Core Product Specifications and Parameters

Biological Background: BMP6 Function and Localization

• BMP6 (bone morphogenetic protein 6), also known as VGR or VGR1, is a secreted growth factor belonging to the TGF-beta superfamily of cytokines.
• It plays essential roles in cartilage and bone formation during development, acting as a key osteogenic and chondrogenic factor. Related references: PMID:31019025
• BMP6 regulates iron metabolism by acting as a ligand for hemojuvelin (HJV) and inducing hepcidin (HAMP) expression, a process mediated through its receptor BMPR1A/ALK3. Related references: PMID:26582087 PMID:30097509 PMID:31800957
• Canonical signaling is initiated by binding to type I receptor ACVR1 and type II receptor ACVR2B, leading to phosphorylation of SMAD1/5/8 and transcriptional regulation. Related references: PMID:18070108
• Non-canonical signaling through the TAZ-Hippo pathway can modulate VEGF signaling by regulating VEGFR2 expression, linking BMP6 to angiogenesis. Related references: PMID:33021694
• The protein contains a signal peptide, a propeptide, and a mature C-terminal growth factor domain; it is glycosylated, disulfide-bonded, and cleaved at paired basic residues for activation.
• Subcellular localization is primarily extracellular (secreted), consistent with its role as a morphogen.

Experimental Guidance and Technical Tips

• The immunogen (aa 375–513) corresponds to the C‑terminal region of BMP6; it may detect both the full‑length precursor and the mature growth factor form.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to visualize secreted or membrane‑associated BMP6 in your cell system.
• For ELISA, validate the detection antibody pair and matrix compatibility in the relevant biological sample (e.g., serum, culture supernatant).
• Reported cross‑reactivity with mouse BMP6 suggests epitope conservation; confirm suitability for other species via sequence alignment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw‑material procurement, offering a seamless supply chain from concept to clinic. This anti‑BMP6 polyclonal antibody supports research in bone development and iron metabolism, and we provide complementary raw materials such as validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our expertise also extends to rare target sourcing and technical consultation to accelerate your diagnostic development.