Anti-BMP6 Monoclonal Antibody for WB, IF/ICC, ELISA - P22004

Core Product Specifications and Parameters

Biological Background: BMP6 Function and Localization

• Bone morphogenetic protein 6 (BMP6), also designated VG-1-related protein (VGR-1), is a member of the TGF-beta superfamily and plays essential roles in cartilage and bone formation. Related references: PMID:31019025
• BMP6 is a key regulator of hepcidin (HAMP) expression and iron metabolism, acting as a ligand for hemojuvelin/HJV and signaling through BMPR1A/ALK3 to promote HAMP transcription. Related references: PMID:26582087 PMID:30097509 PMID:31800957
• It initiates canonical BMP signaling by associating with type I receptor ACVR1 and type II receptor ACVR2B, leading to phosphorylation of SMAD1/5/8 and transcriptional regulation. Related references: PMID:18070108
• BMP6 can also signal through non-canonical TAZ-Hippo pathway to modulate VEGF signaling by regulating VEGFR2 expression. Related references: PMID:33021694
• Subcellular localization: secreted glycoprotein that undergoes cleavage on a pair of basic residues and contains disulfide bonds.
• Keywords: chondrogenesis, osteogenesis, developmental protein, cytokine, growth factor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 350–450 of human BMP6 (NP_001709.1), a region that may be conserved across human, mouse, and rat, consistent with the antibody's reported cross reactivity. Consider sequence alignment to verify epitope conservation.
• For Western blot (WB), a reducing and denaturing sample preparation is recommended to detect the expected ~57 kDa monomer. Non-reduced samples may show higher molecular weight forms due to disulfide bonds.
• In immunofluorescence/immunocytochemistry (IF/ICC), validate antibody specificity using appropriate controls, such as knockdown or overexpression cell lines, to confirm localization.
• For ELISA, the antibody can be explored as a capture or detection reagent in sandwich assays. Pairing with another BMP6-specific antibody may be necessary for optimal performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering this anti-BMP6 monoclonal antibody alongside a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you are developing assays for bone biology, iron metabolism, or TGF-beta signaling, our integrated supply chain and technical support help diagnostic manufacturers and researchers accelerate from concept to clinic. Reach out to explore rare target raw-material sourcing and customized solutions for your next-generation diagnostics.