Anti-BMP5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P22003

Core Product Specifications and Parameters

Biological Background: BMP5 Function and Localization

• BMP5 (bone morphogenetic protein 5) is a secreted glycoprotein and member of the TGF-beta superfamily, encoded by the BMP5 gene. Alternative splicing produces multiple isoforms, and the protein undergoes proteolytic cleavage.
• BMP5 plays essential roles in developmental processes, including cartilage and bone formation and neurogenesis. Related references: PMID:11580864, PMID:29321139
• It initiates canonical BMP signaling by binding to type I receptor BMPR1A and type II receptor BMPR2, leading to phosphorylation of SMAD1/5/8 transcription factors. Related references: PMID:11580864, PMID:29321139
• BMP5 can also signal through the non-canonical MAPK p38 pathway to promote chondrogenic differentiation. Related references: PMID:20402566
• It promotes the expression of HAMP (hepcidin), and this activity is repressed by interaction with ERFE (erythroferrone). Related references: PMID:30097509
• Subcellular localization: BMP5 is secreted and acts as an extracellular signaling molecule.
• Tissue specificity: Expressed in the lung and liver.
• Keywords: Chondrogenesis, osteogenesis, neurogenesis, cytokine, developmental protein, differentiation, disulfide bond, glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen used to raise this antibody corresponds to amino acids 317–454 of human BMP5, a region in the C-terminal portion of the protein. This design may confer target specificity, but cross-reactivity with other BMP family members should be evaluated in relevant assay systems.
• For western blot (WB), a band around 52 kDa is expected; however, actual migration may vary due to post-translational modifications. Use appropriate positive controls (e.g., BMP5-expressing cell lysates or recombinant protein) and validate in the relevant species (human, mouse, rat).
• In immunohistochemistry on paraffin-embedded sections (IHC-P), consider optimizing antigen retrieval conditions and antibody dilution. Tissue sections from lung or liver (where BMP5 is expressed) may serve as positive control.
• For ELISA applications, confirm reactivity against the immunogen or full-length BMP5 protein and establish standard curves with known concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and access to rare target raw materials. Our supply capabilities in bone morphogenetic protein and TGF-beta signaling research ensure you have reliable reagents for your developmental biology and diagnostic development projects.