Anti-BMP4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P12644

Core Product Specifications and Parameters

Biological Background: BMP4 Function and Localization

• BMP4 (bone morphogenetic protein 4), also known as BMP-2B, is a secreted growth factor belonging to the TGF-beta superfamily that plays critical roles in embryonic development, including neurogenesis, vascular development, angiogenesis, and osteogenesis. Related references: PMID:31363885
• BMP4 initiates the canonical BMP signaling pathway by binding to type I receptor BMPR1A and type II receptor BMPR2, leading to phosphorylation and activation of SMAD1/5/8 transcription factors that regulate target gene expression. Related references: PMID:25868050 PMID:8006002 PMID:29212066
• In addition to SMAD-dependent signaling, BMP4 can activate non-canonical pathways such as ERK/MAP kinase, PI3K/Akt, or SRC cascades; for instance, BMP4-induced SRC phosphorylation promotes angiogenesis via VEGFR2. Related references: PMID:31363885
• BMP4 is also involved in odontogenesis and mammary gland development, where it cooperates with MSX1 and PTHLH to regulate epithelial-mesenchymal interactions and ductal outgrowth (by similarity).
• The protein is secreted and localizes to the extracellular matrix, consistent with its role as an extracellular signaling molecule.
• BMP4 is predominantly expressed in the lung and at lower levels in the kidney; it is also present in normal and neoplastic prostate tissues and prostate cancer cell lines.
• Post-translational modifications include glycosylation, disulfide bond formation, and cleavage at paired basic residues to generate the active dimeric form. BMP4 is also phosphorylated, which may modulate its activity.

Experimental Guidance and Technical Tips

• This polyclonal antibody was generated using a synthetic peptide corresponding to amino acids 250-350 of human BMP4 (NP_001193.2). For optimal results, consider validating antibody specificity in your experimental system using positive and negative controls.
• In Western blotting (WB), the expected molecular weight of BMP4 is approximately 47 kDa under reducing conditions. A band near this size may be observed; however, additional bands corresponding to precursor or cleaved forms might appear due to proteolytic processing.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) are recommended to expose the epitope. Optimize antibody dilution and incubation conditions for your specific tissue type.
• In immunofluorescence (IF/ICC), include appropriate controls to distinguish specific staining from background. Permeabilization may be necessary for intracellular staining.
• For ELISA applications, the antibody can be used as a capture or detection reagent. Pair it with a validated matched secondary antibody for detection.
• Due to reported cross-reactivity with mouse and rat, this antibody is suitable for studies in rodent models; however, always confirm reactivity in your target species before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials. Whether you need validated antibody pairs, bulk ancillary reagents, or help sourcing rare targets, our team supports you from concept to clinic. This BMP4 polyclonal antibody exemplifies our commitment to supplying reliable reagents for developmental biology, oncology, and signal transduction research, backed by flexible sourcing and technical consultation.