Anti-BMP3 Polyclonal Antibody for WB, IHC-P, ELISA - P12645

Core Product Specifications and Parameters

Biological Background: BMP3 Function and Localization

• BMP3 (Bone morphogenetic protein 3), also known as BMP-3A or Osteogenin, is a secreted member of the TGF-beta superfamily that plays a critical role in skeletal development and bone homeostasis.
• It acts as a negative regulator of bone density by antagonizing the osteogenic activity of other BMPs, thereby inhibiting osteoprogenitor differentiation and ossification. Related references: PMID:11138004, PMID:15269709
• BMP3 signals through the type II receptor ACVR2B, activating both SMAD2-dependent and SMAD-independent pathways including TAK1 and JNK cascades. Related references: PMID:31665064
• As a secreted glycoprotein, BMP3 contains conserved structural motifs such as disulfide bonds and proteolytic cleavage sites at basic residues, typical of TGF-beta family ligands.
• BMP3 is predominantly expressed in adult and fetal cartilage, consistent with its involvement in chondrogenesis and osteogenesis.
• Key associated keywords include 3D-structure, chondrogenesis, differentiation, growth factor, and osteogenesis, reflecting its essential developmental roles.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 243-472 of human BMP3) corresponds to the mature domain; consider that the antibody may detect both precursor and mature forms depending on sample preparation.
• For Western blotting, a band near 53 kDa is expected under reducing conditions; however, post-translational modifications such as glycosylation may shift the observed molecular weight.
• For immunohistochemistry (IHC-P), validate the antibody in positive control tissues like cartilage or developing bone; antigen retrieval conditions may need optimization.
• The antibody shows cross-reactivity with mouse and rat BMP3, allowing use in rodent models; nevertheless, confirm reactivity with your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with convenient access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For TGF-beta superfamily and bone development research, we offer an integrated sourcing solution that streamlines your procurement of high-quality BMP3 reagents and related products. Whether your project demands rare target antibody sourcing or full-scale raw-material supply, CamelBio supports your journey from concept to clinic.